Furthermore, AAV1-TRB3 shRNA was used to knockdown the expression of TRB3 in pulmonary arteries by previously published methods [22, 23], and TRB3 knockdown reversed and prevented hypoxia-induced pulmonary hypertension, right ventricular hypertrophy and pulmonary arterial remodeling in the hypoxic PH model. Here, TRIB3 is linked to pulmonary arterial hypertension.