The results showed that cytokine receptor interaction, T cell receptor signaling pathway, antigen processing and presentation, chemokine signaling pathway, natural killer cell mediated cytotoxicity, toll like receptor signaling pathway, B cell receptor signaling pathway, and JAK STAT signaling pathway were significantly related to Cluster 2 (Additional file 1: Figure S1), indicating that the clusters containing pyroptosis regulators were associated with tumor immune microenvironment. Here, PPIB is linked to neoplasm.