There are two possible hypotheses that may explain this surprising result: i) the Neurotrace+ area corresponds to the penumbra and ii) NeuN protein is expressed not only by neurons but also by astrocytes[62, 63] (astrocytes are known to be increased after stroke in the infarct and peri‐infarct area[64, 65, 66]). This evidence concerns the gene RBFOX3 and Stroke.