Cell Counting Kit-8 (CCK-8), colony formation, and 5-ethynyl-2′-deoxyuridine (Edu) assays showed that KDM4A-AS1 overexpression notably enhanced HCC cell growth (P < 0.05, Fig. 2A–C and Supplementary Fig. 3A–C), while KDM4A-AS1 knockdown remarkably restrained SK-Hep-1 and MHCC-97H cell proliferation (P < 0.05, Fig. 2A–C and Supplementary Fig. 3A–C). This evidence concerns the gene KDM4A-AS1 and hepatocellular carcinoma.