IFNG and neoplasm: In addition, immunization with plasmids containing genes encoding either wild type or mutant E2 proteins with mutated N-glycosylation sites (N560NT and N576ST) close to these regions were mutated separately or in combination led to significantly higher E2-specific CTL response, IgG2a/IgG1 ratios, expression of IFN-γ, and suppression of tumor growth (P < 0.05) with the E2-M2 mutant (at N576ST) compared to control.