Cardiac morphometrics (Supplementary Table 1), histological assessment of cardiomyocyte diameter (Figures 3A,B and Supplementary Figures 1A,B), and ELISA and immunoblots of hypertrophy biomarkers (ANP and BNP) (Figures 3C,D and Supplementary Figures 1C–E) showed that chronic administration of ISO induced cardiac hypertrophy markedly. Here, NPPB is linked to cardiac hypertrophy.