Constant characterisation of the cell lines via the analysis of typical MM markers (e.g. mesothelin, calretinin, 5T4, podoplanin, cytokeratins, and HBME1), karyotyping and/or short tandem repeat/single-nucleotide polymorphism analysis is important to confirm that they maintain properties consistent with the original tumour subtype (8). Here, MSLN is linked to Miyoshi myopathy.