The treatment with PcrV-primed BMDMs decreased tumor growth (Figure 3C) and weight (Figure 3D) and increased the levels of apoptosis (Figure 3E) and iNOS expression (Figure 3F), and the percentage of iNOS+F4/80+ TAMs (Figure 3F) in tumor tissues; however, PcrV treatment did not affect either tumor growth (Figure 3C) or weight (Figure 3D) in mice depleted of endogenous macrophages due to clodronate liposome administration. This evidence concerns the gene NOS2 and neoplasm.