Here, we further found that NO, in combination with PcrV, activates the PI3K/AKT/mTOR-glycolysis signaling pathway in TAMs, resulting in the formation of a PI3K/AKT/mTOR-glycolysis-NO feedback loop that increases NO generation and, consequently, NO-associated cytotoxicity against cancer cells (Figure 7L). Here, MTOR is linked to cancer.