TNF and hydrops fetalis: Western (trypsin and lipase) and ELISA analysis (BNP, CRP, TNF-a) showed significantly higher protein levels of both trypsin and lipase in the HF patients when compared to Controls in both Cohort 1 and Cohort 2 (p < 0.001 by t-test and KS test) (Figs. 1a and 1b; Supplement Fig. 1S A, B).