The optimal HCC gene panel included DST, SYNE2, APOB, KDM6A, USP9X, SPAG17, TERT, TP53, AMPH, EP300, TTK, CCND1, FCRL1, IL6ST, PTEN, SFN, STK11, and NRAS. This evidence concerns the gene USP9X and hepatocellular carcinoma.