In this study, SH-SY5Y neuroblastoma cells were used to obtain human neuron-like cells using the sequential all-trans retinoic acid (ATRA) differentiation and brain-derived neurotrophic factor (BDNF) maturation program (Encinas et al., 2000; Goldie et al., 2014). The gene discussed is BDNF; the disease is neuroblastoma.