Finally, RNA sequencing of KPM cells comparative to normal pleural mesothelial cells revealed a distinctive transcriptomic signature that included classic mesothelioma markers (Msln, Spp1, Efemp1, Pdpn, Wt1) as well as new candidate mesothelioma genes (Fig 9A–C and Appendix Table S4). Here, MSLN is linked to mesothelioma.