To locate the expression of the TACC1 variants, immunofluorescence assay (IF) was used to detect the expression of TACC1 in HNSCC cell lines and NHOK cells using two kinds of TACC1 antibodies including antibodies which are specific to the TACC1 SPAZ domain and TACC domain. This evidence concerns the gene TACC1 and head and neck squamous cell carcinoma.