To explore the potential function of hypoxia-induced upregulation of Fascin-1 in liver cancer, we constructed two single guide RNAs (sgRNAs) (sg-Fascin-1#1 and sg-Fascin-1#2) specifically targeting Fascin-1 and depleted Fascin-1 expression using the CRISPR/Cas9 system. The gene discussed is FSCN1; the disease is liver cancer.