As shown in Fig. 4A, in breast cancer tissues, the normalized results using SF1 + TRA2 + THRAP3 (R2 = 0.847, P < 0.001), THRAP3 + RHOA + QRICH1 (R2 = 0.947, P < 0.001), SF1 + THRAP3 (R2 = 0.827, P < 0.001), or THRAP3 + RHOA (R2 = 0.866, P < 0.001) displayed a high correlation with SF1 + TRA2B + THRAP3 + RHOA + QRICH1 suggesting that they had extremely similar normalization capabilities. The gene discussed is THRAP3; the disease is breast cancer.