The expression of HIF1A in breast cancer cells was higher (P < 0.01) in MCF-10A and MDA-MB-231 cells than in MCF-7 cells, while no significant difference was found between MCF-10A and MDA-MB-231 cells when using the 3 or 5 RG combinations (SF1 + TRA2B + THRAP3 + RHOA + QRICH1, SF1 + TRA2B + THRAP3, or THRAP3 + RHOA + QRICH1) for normalization (Fig. 3D). This evidence concerns the gene TRA2B and breast carcinoma.