In the neonates with hyperbilirubinemia, the bilirubin level of 74 cases of G6PD deficiency was 334.43 ± 79.27 μmol/L, which was markedly higher than that of neonates with normal G6PD (300.30 ± 68.62 μmol/L). This evidence concerns the gene G6PD and Hyperbilirubinemia.