In order to study the function of MEG3 in HCC, MEG3 of HepG2 and Huh7 cells were upregulated and downregulated separately, RT-qPCR results indicated that compared with the vector group, transfection of MEG3-overexpressing vector significantly enhanced MEG3 expression while knockdown of MEG3 exerted the opposite way (Figure 2(a)). The gene discussed is MEG3; the disease is hepatocellular carcinoma.