Finally, we performed HE staining (Fig. S2A), immunofluorescence staining (Fig. 5a), immunohistochemical staining (Fig. S2B-C), and Western blot analysis (Fig. 5b) on nude mouse tumor tissues to detect protein expression changes in HK2, GLUT1, Ki67, PCNA, CDK2, CyclinD1, Bax, and Bcl-2. Here, HK2 is linked to neoplasm.