To further investigate the role of CENP-N in nasopharyngeal carcinogenesis and development, we generated CENP-N knockdown cell lines (shCENP-N1 and shCENP-N2) with two shRNA sequences in the NPC cell lines 5-8F and CNE-2Z (Fig. 2a-b, P < 0.05). This evidence concerns the gene CENPN and nasopharyngeal carcinoma.