The results showed that the expression of HK2, GLUT1, Ki67, PCNA, CDK2, CyclinD1 and Bcl-2 was downregulated, while Bax expression was upregulated after knockdown of CENP-N in two NPC cell lines in vivo (P < 0.05). The gene discussed is SLC2A1; the disease is nasopharyngeal carcinoma.