The present study also demonstrated that circ_0000326 activated the PI3K/AKT pathway, and miR-338-3p inhibitor or ETS1 overexpression reversed the inhibitory effects of circ_0000326 knockdown on the PI3K/AKT pathway, indicating that circ_0000326 was involved in the regulation of BC progression through the miR-338-3p/ETS1/PI3K/AKT pathway. This evidence concerns the gene AKT1 and breast cancer.