We screened pathways and found that the RPR-FC may be involved in fluid shear stress and the atherosclerosis signaling pathway, the MAPK signaling pathway, the NF-kappa B signaling pathway, the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway, the VEGF signaling pathway, the TNF signaling pathway, and other signaling pathways to exert immunoregulatory and neuroprotective effects after IS. The gene discussed is TNF; the disease is atherosclerosis.