At the higher temperature of 37°C associated with mammalian infection, TLR2 was primarily activated with concurrent suppression of TLR4 activation that coincided with the biosynthesis of tetra- or penta-acylated modified forms of lipid A as previously reported (Kawahara et al., 2002; Rebeil et al., 2004; Montminy et al., 2006; Matsuura et al., 2010). This evidence concerns the gene TLR4 and infection.