The expression of PCIF1 was positively correlated with TMB of MESO, GBM, LUAD, and brain lower-grade glioma (LGG) but negatively correlated with TMB of COAD, THYM, PRAD, THCA, BRCA, and UCEC (p < 0.05). Here, PCIF1 is linked to glioblastoma.