Compared with that in the MG-NAFL, MG-NASH, and MG-fibrosis mice, the expression level of the TRPM4 protein in the GXZY-NAFL, GXZY-NASH, and GXZY-fibrosis mice was decreased, indicating that the Gexia Zhuyu decoction had a good inhibitory effect on the abnormal expression of TRPM4 at all three stages of NAFLD. This evidence concerns the gene TRPM4 and metabolic dysfunction-associated steatohepatitis.