Compared with those of the CG-NAFL, CG-NASH, and CG-fibrosis mice proteins, the MG-NAFL, MG-NASH, and MG-fibrosis mice protein bands were darker and thicker, suggesting that TRPM4 protein expression was abnormally increased in NAFLD. Here, TRPM4 is linked to metabolic dysfunction-associated steatotic liver disease.