Of the 3 HCC lines, HuH-7 cells were chosen for most follow-up experiments, since they are readily transfectable, are responsive to glucose, grow well in culture, are capable of gluconeogenesis and express GLUT2, although GLUT1 is overexpressed as is typical in HCC lines16,17. The gene discussed is SLC2A2; the disease is hepatocellular carcinoma.