TP53 and cancer: To this end, we generated a 141 bp (17:7577010–7577150 in GRCh37, covering a TP53 exon) synthetic ‘WT’ molecule without mutations and a ‘MUT’ insert of the same genomic locus with three cancer hotspot mutations in TP53. Both samples were mixed to obtain a low-abundant mutant sample of 99.9% WT and 0.1% MUT molecules (‘WT/MUT’; Supplementary Fig. 10).