To obtain real-time imaging and additional anatomic information about a tumor, Choi et al. synthesized a kind of iodine containing diatrizoic acid (DTA)-conjugated glycol chitosan (GC) SNP, which was used to physically encapsulate a US imaging agent (perfluoropentane, PFP) via the O/W emulsion method to prepare GC-DTA-PFP nanoparticles [59]. This evidence concerns the gene PRF1 and neoplasm.