Therefore, we chose established cell lines that provide a model system to study leukemia proliferation in vitro by using FLT3-ITD positive (MV4-11 [33], Molm13 [34]) and FLT3-wild type (THP-1 [34], HEL [35], HL-60 [33]), as well as p53-wt (MV4-11 [36], Molm13 [37]) and p53-mutated (THP-1 [38], HL-60 [37], HEL [37]) AML cell lines to analyze the loss of ATG3 on AML proliferation. This evidence concerns the gene FLT3 and leukemia.