To determine whether RANK transduces activating signals into AML cells, primary leukemic cells of patients were incubated with the RANK agonist Fc-hRANKL or isotype control for 24 h, followed by analysis of cytokine levels in the culture supernatants using LEGENDplexTM assays. The gene discussed is TNFRSF11A; the disease is acute myeloid leukemia.