Meanwhile, the results from real-time fluorescence quantitative PCR (qRT-PCR) and Western blot analyses indicate that several pivotal genes of Wnt/β-catenin signaling (e.g., β-catenin, cyclin D1, and c-myc), are remarkably suppressed in CC cells transfected with siRNA targeting for PCAT6 [28]. Here, PCAT6 is linked to cholangiocarcinoma.