HIF1A and cancer: Thereafter, radiation treatment followed by in situ post-radiation monitoring (Appendix A Figure A1) (one day, three days, seven days post-treatment) of the 3D cell cultures took place via quantification of (i) HIF-1a secretion by the cancer cells; (ii) live/dead and apoptotic profiles; and (iii) ECM (collagen-I) secretion by the cancer cells.