We first investigated the methylation of all the promoters of the genes involved in the RASSF/Hippo pathway, specifically RASSF1/2, STRK4/3 (coding for MST1 and MST2 proteins respectively), and LATS1/2, by MS-PCR using DNA extracted from the tumour blocks of 100 patients. This evidence concerns the gene STK3 and neoplasm.