Expression of S100A9 was downregulated in all bladder cancer cells, compared to Urosta 0.16, 2.12, 1.45, and 0.13-fold downregulation in RT4, J82, HT1197, and 253JB-V cells at p-value 0.000 (Figure 9). The gene discussed is S100A9; the disease is urinary bladder cancer.