Nevertheless, for both gene targets the samples with 10 cells spiked in 3 mL gave a significantly higher signal than samples without cancer cells (p = 0.0020 and 0.0045 for USH1C and CKMT1A, respectively, Mann-Whitney test), indicating that the complete assay was able to differentiate between a sample with only few cells and negative sample. Here, CKMT1A is linked to cancer.