To verify the effect of Leptospira on E-cadherin expression, the E-cadherin protein levels in the HK2s and hRPTECs infected with pathogenic L. santarosai serovar Shermani (L. santarosai) and non-pathogenic L. biflexa serovar Patoc (L. biflexa) at a multiplicity of infection (MOI) of 100 were examined using western blotting. The gene discussed is CDH1; the disease is infection.