The expression of EMT markers such as vimentin, twist, Zinc finger E-box-binding (ZEB)1, ZEB2, snail, slug, and E-cadherin has been studied in liver-derived CTCs from HCC patients, being the co-expression of twist and vimentin significantly correlated with tumor burden, macrovascular invasion, and more advanced stages [86]. Here, TWIST1 is linked to hepatocellular carcinoma.