DNM2 and Duchenne muscular dystrophy: Troponin T immunostaining (a cytoskeletal protein expressed exclusively in myotubes) and nuclei labeling showed a similar mean number of nuclei in cultured myotubes from healthy boys (controls, n = 5) and boys with DMD (n = 6) (Figure 1A) (481.7 ± 37 nuclei/mm2 and 445.8 ± 55 nuclei/mm2, respectively, p = 0.6), but a reduction in the myotube area (Troponin T expressing cells; 0.518 ± 0.036 mm2 and 0.394 ± 0.033 mm2, p = 0.035) and fusion index (nuclei per myotube/total nuclei; 76.8 ± 2.9% and 66.2 ± 2.3%, p = 0.005) in DMD samples (Figure 1B).