Examination of RNA-seq data from 28 samples comprising two canine B lymphoid cell lines, two T lymphoid cell lines, primary B-cell lymphomas, and non-neoplastic controls revealed frequent potentially loss-of-function variants among some of the genes encoding the primary targets of AS-605240 and ibrutinib and their regulators: BTK, PI3K, and PTEN. Here, PIK3CG is linked to B-cell non-Hodgkin lymphoma.