To this end, Bie et al. (2011) conducted quantitative polymerase chain reaction analysis on RNA from 6 normal brain samples and 38 gliomas with survival time data and found that the expression of Bub3 mRNA was associated with tumor grade and prognosis in glioma tumors: with the increase of WHO classification, Bub3 gene expression significantly increased. Here, BUB3 is linked to central nervous system cancer.