Transient Treg-cell depletion also increased IFN-γ production non-specifically from CD3- cells including NK cells, as we showed previously that Treg-cell depletion induced effective anti-tumor immunity by inducing tumor-specific cytotoxic CD8+ T cells and IFN-γ-producing CD4- CD8- cells, including NK cells [15]. The gene discussed is CD8A; the disease is neoplasm.