NOS2 and Anxiety: These results were consistent with the reported anxiolytic effects of NOS inhibitors [18, 19] and compatible with the reports of Bonassoli et al. [22] and Gonzaga et al. [23] revealing that infusion of a nonselective NOS inhibitor, a selective nNOS inhibitor, or a selective inducible NOS (iNOS) inhibitor (N-([3-(aminomethyl)phenyl]methyl) ethanimidamide dihydrochloride) into the dorsolateral periaqueductal gray matter or into the dorsal raphe nucleus, respectively, attenuated EtOHW anxiety in rats 24 or 48 hours after discontinuation of EtOH.