In addition, although there is a lack of direct evidence that arsenic trioxide exerts anti-HCC effect by the estrogen signaling pathway, thyroid hormone signaling pathway, focal adhesion, and sphingolipid signaling pathway, studies have reported that these pathways are extremely related with HCC [47–50] and have higher rich factor in KEGG analysis, which indicates that these pathways might be the potential anti-HCC pathways of arsenic trioxide and points out the way for our future research. Here, TG is linked to hepatocellular carcinoma.