To further determine if heterozygotes of ABCG5/ABCG8 genes could drive hypercholesterolemia and macrothrombocytopenia phenotypes, we created a double knockout rat (Figure 3A), having a growth curve similar to the WT strain without visible abnormalities (Supplementary Figure S4A) and succesful depletion of ABCG5 and ABCG8 expression in liver and intestine (Supplementary Figure S4B). This evidence concerns the gene ABCG8 and Macrothrombocytopenia.