CCL4 and asthma: Next, to determine whether experimental asthma attenuates the increase in Ccl4 and Ccl5 expression observed in the optic nerves of Nf1OPG mice at 12 (Fig. 2c and Supplementary Fig. 3b, c) and 24 (Supplementary Fig. 3d, e) weeks of age, quantitative real-time PCR was performed.