Next, to evaluate the effect of endogenous miR-675 on endogenous DUX4, we transfected 15A FSHD myoblasts with anti-miR-675 or anti-miR -ctrl, differentiated cells for 4 days, and then used immunofluorescence to detect DUX4 protein. This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.