DUX4 and facioscapulohumeral muscular dystrophy: To evaluate the effect of endogenous miR-675 on the levels of transfected DUX4, we performed western blot on proteins extracted from 15 A FSHD cells transfected with V5-tagged DUX4-FL or miR-675-resistant DUX4 (DUX4-miR-675Res) expression plasmids with and without anti-miR-675.