When challenged with high-fat diet (HFD), Gpr180 knockout mice gained more weight (Fig. 2g) and this was accompanied by higher fat mass accumulation (Supplementary Fig. 2j and k), pronounced liver steatosis and increased plasma ALT activity indicating an early stage of liver disease (Fig. 2h, Supplementary Fig. 2l and m). This evidence concerns the gene GPR180 and liver disorder.