Moreover, our work demonstrated that CRTC1 deficiency contributes to BBB destabilization by increasing Claudin-1 expression in BMEC after ischemia/reperfusion for the first time using immunofluorescence staining, and that inhibition of Claudin-1 by miR-132 improves BBB recovery after ischemia/reperfusion. This evidence concerns the gene CRTC1 and ischemia.