However, we observed no reduction in IC50 values for PfPDK1 depleted (−Shield-1) compared to NF54/PDK1 cKD control parasites (+Shield-1) or compared to NF54 WT parasites cultured in the presence or absence of Shield-1 (S14 Fig), suggesting that all 3 inhibitors are not specific for PfPDK1 but likely target additional/other essential parasite kinases. Here, PDK1 is linked to chronic kidney disease.