MEN1 and cancer: MEN1+/+ and MEN1−/− mouse embryo fibroblasts, a kindly gift from Philippe Bertolino and Chang X. Zhang (Cancer Research Center of Lyon, France) [20], were grown in DMEM (Gibco) supplemented with 10% fetal bovine serum, penicillin (100 Units/ml) and streptomycin (100 μg/ml) at 37 °C in a humidified atmosphere containing 5% CO2.