As the IL-6/JAK/STAT3 signaling pathway is well established to play an important role in a variety of malignancies by promoting proliferation and inhibiting apoptosis of cancer cells (18, 26–29), Western blot was performed to examine whether the related key markers in this signaling pathway changed in H292, A549, and Hcc827 cell lines cultured with different CM. This evidence concerns the gene STAT3 and cancer.