These clusters could be assigned to nine known cell lineages through marker genes, including marked with carcinoembryonic antigen-related cell adhesion molecules 5 (CEACAM5), epithelial cell adhesion molecules (EPCAM) +, Keratin 18 (KRT18) + and transmembrane glycoprotein Mucin 1 (MUC1) + 4 types of CRC cells, colon goblet cell, myeloid-derived monomacrophage, dendritic cells (DC), T lymphocytes, stem cells, B lymphocytes, macrophage, epithelial-mesenchymal transition (EMT) -like fibroblasts, EMT, cancer-related fibroblasts (CAFs) and enterocyte. The gene discussed is CEACAM5; the disease is cancer.