In this study, Smart-seq2 analysis shows that, KRT8, 18 and 19 genes were enriched in the tumor tissues of CRC, and the high expressions of KRT18 and KRT19 genes were significant differences in DES and DS or ES, ES and DS classification, and the high expressions of KRT8 gene has significant differences between DES and ES or DES and DS in patients with CRC. This evidence concerns the gene KRT8 and neoplasm.